HLA class I and II genotyping by PCR-SSO in patients with type-1 diabetes mellitus

Type 1 diabetes mellitus is a chronic autoimmune disease that involves destruction of the pancreatic beta cells. It is well known that both genetic and environmental factors involved in pathogenesis of type-1diabetes mellitus. This study seeks to determine whether there is any association between human leukocyte antigen class I and II alleles and type-1diabetes mellitus. Seventy type-1 diabetes mellitus patients compared to 30 apparently healthy individual were enrolled in this study. Human leukocyte antigens genotyping were analyzed by polymerase chain reaction sequence specific oligonucleotide technique. The present study revealed significantly high frequency of DQB1*0101 and DQB1*0201 alleles among patients in comparison with healthy control, while there was significantly low frequency of each HLA-A*3301, B*0826, DRB1*0701, *1101 and HLA-DQB1*0604 alleles in patients as compared to healthy controls. These findings indicate that higher frequency of HLA-DQB1*0101 and *0201 alleles may be a risk factor for type-1diabetes mellitus, meanwhile low frequency of HLA-A*3301, B*0826, DRB1*0701, *1101 and HLA-DQB1-*0604 alleles could be a protective factor

Preoperative and Postoperative Serum Immunoglobulins Levels in Colorectal Cancer of Iraqi Patients

Quantitation of serum immunoglobulins [IgG, IgA and IgM] provides useful information for the evaluation of certain cancers. to estimate serum immunoglobulins level before and after surgery, and to shed light on the correlation of immunoglobulins with progression of CRC. By single radial immune diffusion method IgG, IgA, IgM were estimated in 100 CRC patients preoperatively, and in 20 patients postoperatively compared with 35 patients control with ulcerative colitis [UC] and 50 healthy control. The study showed significantly increased the serum IgM level in patients group preoperatively [p < 0.001] compared with control group. On the other hand, no significant differences were observed in respect to the mean of IgG, IgA, and IgM after surgical excision of the tumor in 20 patients. These finding suggests that high level of IgM might be considered as prognostic indicator

Possible association of HLA-DR and DQ molecules with colorectal cancer in Iraqi patients

Human leukocyte antigen [HLA] is the most polymorphic genetic system in man. The genes of this region influnence susceptibility to certain diseases. This study was established to shed light on the possible association of HLA class-II antigens and CRC patients, and to correlate the findings with both family history and tumor location. Microlymphocytotxicity assay has been used to assess HLA-typing of 150 blood samples of 100 CRC patients and 50 healthy normal control. An increased frequency of HLA-DR7 was observed for patients' group versus control group with P-value [<0.005], moreover, statistical analysis showed non significant correlation of HLA-DR7 with both family history of CRC and tumor location. This finding demonstrate that HLA-DR7 might play a role in CRC susceptibility

Possible association of HLA class-1 molecules with colorectal cancer in Iraqi patients

Genetic factors were found to play a crucial role in the development of colorectal cancer. This study was established to shed light on the possible association of HLA class-1 antigens and CRC patients, and to correlate the findings with both family history and tumor location.. Lymphocytotxicity assay has been used to assess HLA-typing of 150 blood samples of 100 CRC patients and 50 healthy normal controls in College of Dentistry/ University of Baghdad. Comparison between CRC patients and healthy controls showed several antigens, deviations in their frequencies. HLA-A2, A28 and B39 antigens were observed with increased frequencies in patient's group with significant differences [P< 0.008, 0.011 and 0.023 respectively; moreover, yet however statistical analysis showed non significant correlation of these specific HLA-Ags with both family history of CRC and tumor location. This finding demonstrated that HLA A2, A28 and B39 might play a role in CRC susceptibility